• Salma Aslam Kundi
  • Mary Forsling
  • Haluck Kelestimur


ABSTRACTPrevious studies have shown that surgical ovariectomy of the rat results in a fallin the plasma visopressin concentrations suggesting that ovarian steroids may influencehormone release and after surgical ovariectomy the response to hypertonicity wasunaffected but that to hypovolemia was attenuated. To determine whether a similar fall isobserved on suppression of the oestrous cycle, vasopressin concentrations were monitoredafter treatment with the antiestrogen preparation tamoxifen. Treatment with tamoxifen wasfound to result in a fall in circulating vasopressin concentrations, with little effect on fluidbalance.To determine whether the ovary could influence the vasopressin release in response toknown stimuli, hormone concentrations were measured in tamoxifen ovarectomized ratsduring extracellular fluid hypertonicity produced by Intra-peritoneal (i.p.) injection ofhypertonic saline and hypovolemia produced by i.p. injection of polyethylene glycol. It wasfound that after treatment with tamoxifen, the response to hypertonicity was unaffected butthat to hypovolemia was attenuated. Hence tamoxifen is a potent anti-estrogen drug, andtamoxifen ovariectomized rats behaved in the same manner as surgically ovariectomizedrats.


Baylis, P.H., Spruce, BA. & Brud, T. (1985). Osmoregulation of vasopressin secretion during the

menstrual cycle. In vasopressin pp.241-247. Ed. R.W. Schrier, New York: Raven Press.

Forsling, M. L., Akerlund, M. & Stromberg, P. (1981). Variations in the plasma concentrations of

vasopressin during the menstrual cycle. "Journal of Endocrinology 89",263-266.

Forsling. M.L. & Peysner K.(I981). Pituitary and plasma vasopressin concentrations and fluid

balance throughout the oestrous cycle of the rat. "Journal of Endocrinology 117"397^102.

Al-Sendi, A., Forsling, M.L., Khan, M. & Peysner, K., (1985). Fluid balance in cycling rats and

ovariectomized rats treated with oestradiol benzoate "Journal of Physiology371" 189P.

Rhodes, C.H., Morrell.J. L & Plaff 'D. W. (1981). Distribution of oestrogen concentrating

neurophysin containing magnocellular neurons in the rat hypothalamus as demonstrated by a

technique combining steroid autoradiography and immunohistology in the same issue.

“Neuroendocrinology 33", 18-23.

Pelletier, G., Liao N, Follea N & Govindan. (1988). Mapping of oestrogen receptor producing cells in

the rat brain by in situ hybridization. "Neuroscience Letters 94", 23-28.

Crofton J.T., Ratliff LDL., Brooks, DP. & Share, L. (1986) The metabolic clearance rate and presser

responses to vasopressin in male and female rats. "Endocrinology 118", 1777-1781.

Ferris, T.F. & Francisco, LL. (1983). Estrogen, progesterone and kidney. In "Renal Endocrinology"


Thomborough, J.R. &Passo, S.S., (1975). The effects of estrogen on sodium and potassium metabolism

in rats. "Endocrinology 97" 1528-1538.

Wathes, D.C. (1984). Possible actions of gonadal oxytocin and vasopressin. "Journal of Reproduction

and Fertility 71", 315-345.

Furr J.A. & Jordan, V.C. (1984). The pharmacology and clinical uses of tamoxifen. "Pharmacology

and Therapeutics 25 "201-205.

Forsling, M.L., Matziari, & Aziz, L. (1988). A comparison of the vasopressin response of rats to

intraperitoneal and intravenous administration of hypertonic saline and the effect of opioid and

aminergic antagonists. "Journal of Endocrinology 345" 217-224.

Forsling.M.L.& Peysner, K. (1983). A method for studying hormone release during hypovolemia in

the rat. "Journal of Endocrinology 345 " 5P.

Hussain, M L.J Manger, W.M„ Rock, T.W., Weiss, R.J.& Frantz, A.G., (1979). Vasopressin release

due to manual restraint in the rat, role of body compression and comparison with other stressful

stimuli. "Endocrinology 104 ". P641-644.

De-Vries, G.J. Buijs, R.M.& Sluiter AA. (1984). Gonadal hormone actions on the morphology of the

vasopressinergic innervation of the adult rat brain. “Brain Research 298",141-145.

Skowsky; W.R., SwanX. & Smith. (1979). Effects of sex steroid hormones on arginine vasopressin

in intact and castrated male and female rats. “Endocrinology 104 "105-108.

Crofton, J.T. Baer, P.G. Share, Brooks, D.P. (1985). Vasopressin release in male and female rats.

Effect of gonadectomy and treatment with gonadal steroid hormones. “Endocrinology 117", 1195-

Durr J A, Stamoutsos, B. & Lindheimer, M.D. (1981). Osmoregulation during pregnancy in the rat,

evidence for resetting of the threshold for vasopressin secretion during gestation. "Journal of Clinical

Investigation 68",337-346.

Beale, K.J., Duffet, J.M., Forsling, M.L. & Peysner, K.(1986). Observations on the effect of

progesterone on fluid balance and vasopressin metabolism in the rat. “Journal of Physiology


Lindheimer, M.D., Barron, W.M. & Davison, J.M. (1985). Water metabolism and vasopressin

secretion in pregnancy. "Vasopressin" 229-240.

Crofton, J.T., & Share, L. (1989). Osmotic control of vasopressin in male and female rats. "American

Journal of Physiology 257" R738-R743.

Williams, T.D.M., Carter, D.A.& Lightman, S.L. (1985). Sexual dimorphism in the posterior

pituitary' response to stress in the rat. "Endocrinology 116", 738-740