CHANGES IN GLYCOSYLATED PROTEINS IN TYPE-2 DIABETIC PATIENTS WITH AND WITHOUT COMPLICATIONS
Abstract
Background: Diabetes mellitus constitutes one of the most important problems in developing anddeveloped countries. Increased glycosylation of various proteins in diabetic patients has beenreported by many authors. The present study describes the changes in protein glycosylation indiabetic patients with and without diabetic complication. Methods: The study included onehundred and three subjects. Among them 21 were type 2 diabetic patients without any clinicalevidence of chronic diabetic complications, 21 were type 2 diabetic patients with cardiovascularcomplications, 20 were type 2 diabetic patients with cataract, 20 were type 2 diabetic patients withretinopathy and 21 apparently normal, age, sex and weight matched controls. The patients wereselected from Ziauddin Medical University Hospital, Karachi and Jinnah Postgraduate MedicalCentre, Karachi. Results: Fasting plasma glucose was increased in all diabetic patients andcorrelated significantly with glycosylated hemoglobin, glycosylated plasma proteins and serumfructosamine concentrations. There was no significant difference in the levels of fasting plasmaglucose, glycosylated plasma proteins, glycosylated hemoglobin, serum fructosamine, hexosamineor sialic acid between diabetic patients with or without chronic complications. Alpha-1 and alpha-2 globulin fraction were significantly increased in diabetic patients without complications, diabeticpatients with cardiovascular complications and diabetic patients with cataract. Albumin was foundto be decreased in diabetic patients with cataract while gamma globulin was increased in diabeticpatients with cardiovascular complications and diabetic patients with cataract. Conclusions: Inuncomplicated diabetic patients alpha-1 and alpha-2 glycoproteins were increased. In diabeticpatients with cardiovascular complications alpha-1, alpha-2 and gamma globulin were increasedwhile in diabetic patients with cataract alpha-1, alpha-2 and gamma globulin were increased butserum albumin was significantly decreased.Key words: glycosylated hemoglobin, glycosylated plasma protein, serum fructosamine, sialicacid, hexosamine and total serum proteins.References
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