CORNUCOPIA OF CULTURED SCHWANN CELLS: WHERE IS THE BEST SOURCE IN ALBINO RATS?

Authors

  • M. MIRAJULLAH
  • SHEN XINYA
  • LIU C AIDONG
  • Wang Yujiang

Abstract

Background: Schwann Cells (SC) arc multifunctional glia cells with diversified biological potentials. To study theirbiological activities, ample amount of cultured SC is a pre-requisite. Many sources have been exploited in the past forSC culture but unfortunately no attempt has been made to find the richest source. Methods: To make an attempt inthis direction, we cultured SC under identical conditions from various neural sites in the neonate & young SD albinorats. Fresh, cryopreserved & pre-stimulated neural tissue- have been compared for total & % of SC/unit tissue fromthe combined data of living & immunostained cultures Results: Our data shows that the upper limb nerves have notonly quantitative advantage (2.35x104 cells/rng tissue) but also have significant qualitative edge (P< .05- .01) over theremaining sources. It can be concluded from our data that upper limb nerves are the best available source for SC/unittissue.

References

Schwann T. Mikroskopische unlersuch-ungen uber die

uebereinsfimmung in der struktur und dem wachstun der here

und p flanzen. Sander. Berlin. 1839

Askanas V. Engel WK, Dalakas MC. Lawrence JV, & Carter

LS Human SC m (issue culture Histochemical &

ultrastructural studies Arch Neurol. 1980; 37 329-37

Bhattacharyya A, Bracken bury R. & Partner N Neuron-SC

signals are conserved across species: purification and

characterization of embryonic chicken SC. J Neurosci. 1993.

M3

Dupm I: SC differentiation in clonal cultures of the neural

crest. as evidenced by the anti-SC myelin pmlem mAb PNAS

USA 1990; 87; 1119-23.

Eccleston PA. Mirsky R &. Jessen KR lypc-1 collagen

preparations inhibit DNA synthesis in glial cells of the PNS

Exp Cell Res. 1989. 182 1-173-85

Moretto (i. Kim SU, Shut DH. Pleasure DC. and Rizzuto N

Long term cultures of human adult SC isolated front mil tipsy

materials Acta Neurop tol. 1984. 64 15-21.

Wood 'W Separation of functional SC and neurons from

normal peripheral nerve tissue Brain Res. 1976; 115. 361-75

Gue’nard V. Kleitman N. Morrissey TK. Bunge RP. and

Aebischer P Syngeneic SC derived from adult nerves seeded

in semipermeable guidance channels enhance peripheral nerve

regeneration J Neurosci 1992; 12(9) 3310-20

Maeda T. Mackinnon SE. Best 1J cl al Regeneration across

"stepping Stone" nerve grads. Brain Res. 1993; 618(2) 196-

Oda Y, Okada Y, Katsuda S, Ikeda K. & Nakanishi I A simple

method for the SC preparation from newborn rat SN .1

Neurosci Methods! 1989. 28 163-9

Porter S, Glaser I. & Bunge RP Release of autocrine growth

actor by primary and immortalized SC Proc Natl Acad Sci

USA. 1987. 84 7768-72

Raft MC, Hornhy-Smiih A. & Brockes JP cAMP as a

mitogenic for cultured rat SC. Nature. 198”: 273 672-3

Brockes. IP. Fryxell KJ & lemke Oh Studies on SC culture &

morphology J Exp Biol. 1981.95 215-30

Silam V Pizzuti A. Strada D cl al. Human neuronal cell

viability demonstrated in culture after preservation Brain Res.

437 169- 74.

Salzer JL. & Bunge RP. Studies of SC proliferation. (luring

development, Wallerian degeneration. direct injury J Cell

Biol. 1980; 84 739-52.

Murry MR, &. Stout AP Characteristics of human SC in vitro

Anat Rec. 1910: 84: 29543.

Wrathall JR, Rigamonti DO. & Kao CC Cultures

enriched in Schwann-like cells from nerve segment of the

adult eat Brain Res. 1981; 224:218-23.

Mirajullah M Studies on SC cultures; Best source, substrate

dependent morphological characteristics & proliferation in SD

albino rats. PhD thesis. Shanghai Medical University. 1995

Scarpini E, Kreider BQ, Lisak RP et al. Culture of human SC

isolated from fetal nerves. Brain Res, 1988; 440: 261-6.

Scarpini K, Kreider BQ Lisak RP. & Pleasure PH

Establishment of SC cultures from adult rat peripheral nerves.

Exp Neurol. 1988. 102 167-76.

21 In no M, & Suzuki K Isolation and morphologic

characterization in vitro of SC' of the Twitcher mouse. Lab

Invest.1990; 62. 704-12.

Mason PW. Attema BL, & DeVries GH Isolation and

characterization of neonatal SC from cryopreserved rat SN. J

Neurosci Res. 1992; 31. 731-44.

Rutkowski L. Tannekoon GI. & McGillheaddy.JE; Selective

culture of mitotically active human SC’s from adult sural

nerves Ann Neurol, 1992; 31(6): 580-6

Raff MC. Abney I. Brokes JP. & Hornby-Smith A SC growth

factors. Cell, 1978, 15: 813-22.

Rainer N, Glaser L. and Bunge RP. PC-12 as a source of

neurite- derived cell surface mitogen, which stimulates SC

division. J Cell Biol. 150-5

fields Kl. & McMenammP SC cultured from adult rats contain

a cytoskeletal protein related to astrocyte filaments Dev Brain

Res. 20 259-69.

Morrissey TK, Kleitman N, Goddard & M, Aebischer P SC

differentiation in vitro Differences between cells from adult rat

and human nerves. Soc Neurosci Abstr, 1991; 17 376.

Boyer PJ. Rutkowski L, Tuite G el al. Isolation of human SC

from pediatric dorsal nerve roots from dorsal rhizotomy A

adult SN from autopsy Soc Neurosci Absf.,1992; 18:1490

Brockes JP. Fields Kl. & Raff MC A surface antigenic market

for rat SC Nature (London), 1977,266:364-66.

Kim SU. Yong VW, Watabe K. & Shin DH Human fetal SC

in culture: Phenotypic expression & proliferative capability. J

Neurosci Res, 1989; 22: 50-9

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