EFFECTS OF NICOTINE ON AN IN VITRO RECONSTITUTED MODEL ORAL MUCOSA IN TERMS OF CYTOKINE PRODUCTION

Authors

  • Muhammad Nauman Sheikh
  • Sajid Hanif
  • Majid Zia
  • Zahur Qayyum

Abstract

Background: The extensive use of tobacco and its associated problematic health issues have been aconcern to mankind. The World Health Organization (WHO) estimates that approximately one-third ofthe global population aged 15 years or older are smokers and each smoker consumes an average of 15cigarettes daily. The objective of this study was to establish the effect of nicotine on an in vitroreconstituted oral mucosa model, the effect of treatment with this compound was measured in terms ofcytokine production. Method: Observational laboratory based study design was used to carry out theexperiment. The reconstituted human epithelium model used in the study was prepared and supplied bySkin Ethic Laboratories, Nice, France. The effect of nicotine on epithelial cytokine production wasassessed using commercially available assay kits (R&D systems). This was done using the enzymelinked immuno-sorbent assay. Result: In this study there was evidence that after 5 minutes treatment onun-inflamed mucosa with nicotine at 10 mm concentration GM-CSF release decreased, and also after 24hours treatment with nicotine at 10mM concentration GM-CSF release increased. TNF-α increasedrelease of pro-inflammatory cytokines, IL-6, IL-8, and also GM-CSF from the model mucosa after 24hours, but had no effect on the release of IL-1α., IL-6, IL-8, and GM-CSF after 5 minutes and 24 hoursrespectively. Conclusion: In conclusion at all the concentrations used in this experiment, nicotine had noeffect on the TNF-α stimulated tissue and un-inflamed mucosa and had no significant effect on cytokinerelease including IL-1α., IL-6, IL-8, and GM-CSF after 5 minutes and 24 hours respectively.Keywords: Nicotine, oral mucosa, cytokine

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Published

2011-12-01

Issue

Vol. 23 No. 4 (2011): JOURNAL OF AYUB MEDICAL COLLEGE, ABBOTTABAD

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ORIGINAL ARTICLE

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