SYNERGISTIC ANTIBIOIFLM ACTIVITY OF PROBIOTIC LACTOBACILLUS ACIDOPHILUS AND PUNICA GRANATUM L., AGAINST PSEUDOMONAS AERUGINOSA BIOFILM
DOI:
https://doi.org/10.55519/JAMC-02-12876Keywords:
Biofilm, pseudomonas aeruginosa, Punica Granatum L., lactobacillus acidophilus, 96 well microtiter plate synergismAbstract
Background: Antibiotic resistance is one of the most urgent public health concerns. Biofilm formation is well linked with chronic wounds, chronic obstructive pulmonary disease, urinary tract infections, and cystic fibrosis. Our goal was to assess the biofilm activity of P. aeruginosa and the individual and combined anti-biofilm forming activity of probiotic Lactobacillus acidophilus and Pomegranate peel extract Punica granatum L., against P. aeruginosa. Methods: A total of 150 swabs of urine, blood, pus, and CSF were collected from PNS Shifa Hospital Karachi, and P. aeruginosa was isolated and identified according to standard bacteriological methods. The ability of P. aeruginosa to form biofilms was assessed using a microtiter plate assay. Results: The anti-biofilm forming activity of pomegranate peels extract against P. aeruginosa was 29.26±19.09 whereas the anti-biofilm forming activity of Lactobacillus acidophilus against P. aeruginosa was 0.5×106. When used in combination, there was significant synergistic activity between Punica granatum L. (pomegranate peel extract) and Lactobacillus acidophilus. Conclusion: The unique synergistic mixture of natural product extracts and probiotics has demonstrated more efficiency against rapidly evolving pathogens, serving as promising candidates for developing biofilm inhibitors and perhaps proving as possible environmentally friendly agents against bacteria that produce antibiotic-resistant biofilms.References
Abdulhaq N, Nawaz Z, Zahoor MA, Siddique AB. Association of biofilm formation with multi drug resistance in clinical isolates of Pseudomonas aeruginosa. EXCLI J 2020;19:201–8.
Ahani Azari A, Fozouni L. Incidence of Multidrug-Resistant, Extensively Drug-Resistant, and Pandrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical Specimens. Infect Epidemiol Microbiol 2020;6(3):211–7.
Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules 2020;25(6):1340.
Gebreyohannes G, Nyerere A, Bii C, Sbhatu DB. Challenges of intervention, treatment, and antibiotic resistance of biofilm-forming microorganisms. Heliyon 2019;5(8):e02192.
Avershina E, Shapovalova V, Shipulin G. Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy. Front Microbiol 2021;12:707330.
Botelho J, Grosso F, Peixe L. Antibiotic resistance in Pseudomonas aeruginosa - Mechanisms, epidemiology and evolution. Drug Resist Updat 2019;44:100640.
Heidari R, Farajzadeh Sheikh A, Hashemzadeh M, Farshadzadeh Z, Salmanzadeh S, Saki M. Antibiotic resistance, biofilm production ability and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa strains isolated from nosocomial infections in southwestern Iran. Mol Biol Rep 2022;49(5):3811–22.
Hasan SA, Najati AM, Abass KS. Prevalence and antibiotic resistance of "pseudomonas aeruginosa" isolated from clinical samples in Kirkuk City, Iraq. Eurasia J Biosci 2020;14(1):85–94.
Jackson N, Borges CA, Tarlton NJ, Resendez A, Milton AK, de Boer TR, et al. A rapid, antibiotic susceptibility test for multidrug-resistant, Gram-negative bacterial uropathogens using the biochemical assay, DETECT. J Microbiol Methods 2021;182:106160.
Litwin A, Rojek S, Gozdzik W, Duszynska W. Pseudomonas aeruginosa device associated–healthcare associated infections and its multidrug resistance at intensive care unit of University Hospital: polish, 8.5-year, prospective, single-centre study. BMC Infect Dis 2021;21:1–8.5.
Pang Z, Raudonis R, Glick BR, Lin TJ, Cheng Z. Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnol Adv 2019;37(1):177–92.
Khadraoui N, Essid R, Jallouli S, Damergi B, Ben Takfa I, Abid G, et al. Antibacterial and antibiofilm activity of Peganum harmala seed extract against multidrug-resistant Pseudomonas aeruginosa pathogenic isolates and molecular mechanism of action. Arch Microbiol 2022;204(2):133.
Melander RJ, Basak AK, Melander C. Natural products as inspiration for the development of bacterial antibiofilm agents. Nat. Prod. Rep 2020;37(11):1454–77.
Mishra R, Panda AK, De Mandal S, Shakeel M, Bisht SS, Khan J. Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens. Front Microbiol 2020;11:566325.
Newman DJ, Cragg GM. Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. J Nat Prod 2020;83(3):770–803.
A Elbadri S, S Fathi M, E Abdel Hamid A, A ElGendy H. The effect of Lactobacillus acidophilus as a probiotic against Pseudomonas aeruginosa growth and biofilm formation. Nov Res Microbiol J 2019;3(4):428–39.
Sorrenti V, Randazzo CL, Caggia C, Ballistreri G, Romeo FV, Fabroni S, et al. Beneficial Effects of Pomegranate Peel Extract and Probiotics on Pre-adipocyte Differentiation. Front Microbiol 2019;10:660.
Yi EJ, Kim AJ. Antimicrobial and Antibiofilm Effect of Bacteriocin-Producing Pediococcus inopinatus K35 Isolated from Kimchi against Multidrug-Resistant Pseudomonas aeruginosa. Antibiotics (Basel) 2023;12(4):676.
Gad GF, El-Feky MA, El-Rehewy MS, Hassan MA, Abolella H, El-Baky RM. Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients. J Infect Dev Ctries 2009;3(5):342–351.
Hawas S, Verderosa AD, Totsika M. Combination Therapies for Biofilm Inhibition and Eradication: A Comparative Review of Laboratory and Preclinical Studies. Front Cell Infect Microbiol 2022;12:850030.
Parkins MD, Gregson DB, Pitout JD, Ross T, Laupland KB. Population-based study of the epidemiology and the risk factors for Pseudomonas aeruginosa bloodstream infection. Infection 2010;38:25–32.
Tariq M, Ansari F, Rafiq Mu AA, Arshad F, Nayab A. Evaluation of Multidrug Resistance in Biofilm Forming Pseudomonas Aeruginosa Isolated from Urinary Catheter Tips in Patients from ICU and CCU in DHQ Teaching Hospital Gujranwala. PJMHS 2020;14(4):1980–4.
de Campos FP, Felipe-Silva A, de Melo AC, Passadore LF, Guida SM, Balabakis AJ, et al. Community-acquired Pseudomonas aeruginosa-pneumonia in a previously healthy man occupationally exposed to metalworking fluids. Autops Case Rep 2014;4(3):31–7.
Qin S, Xiao W, Zhou C, Pu Q, Deng X, Lan L, et al. Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics. Signal Transduct Target Ther 2022;7(1):199.
Rewatkar AR, Wadher BJ. Staphylococcus aureus and Pseudomonas aeruginosa biofilm formation methods. J Pharm Biol Sci 2013;8(5):36–40.
Azimi S, Kafil HS, Baghi HB, Shokrian S, Najaf K, Asgharzadeh M, et al. Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. GMS Hyg Infect Control 2016;11:Doc04.
Abdelraheem WM, Abdelkader AE, Mohamed ES, Mohammed MS. Detection of biofilm formation and assessment of biofilm genes expression in different Pseudomonas aeruginosa clinical isolates. Meta Gene 2020;23:100646.
Chen R, Yang S, Zhang L, Zhou YJ. Advanced Strategies for Production of Natural Products in Yeast. iScience 2020;23(3):100879.
Sahoo B, Panigrahi LL, Das RP, Pradhan AK, Arakha M. Biogenic synthesis of silver nanoparticle from Punica granatum L. and evaluation of its antioxidant, antimicrobial and anti-biofilm activity. J Inorg Organomet Polym Mater 2022;32(11):4250–9.
Salim A, Deiana P, Fancello F, Molinu MG, Santona M, Zara S. Antimicrobial and antibiofilm activities of pomegranate peel phenolic compounds: Varietal screening through a multivariate approach. J. Bioresour Bioprod 2023;8(2):146–61.
Nostro A, Guerrini A, Marino A, Tacchini M, Di Giulio M, Grandini A, et al. In vitro activity of plant extracts against biofilm-producing food-related bacteria. Int J Food Microbiol 2023;8(2):33–9.
de Almeida Rochelle SL, Sardi JD, Freires IA, de Carvalho Galvão LC, Lazarini JG, de Alencar SM, et al. The anti-biofilm potential of commonly discarded agro-industrial residues against opportunistic pathogens. Ind Crops Prod 2016;87:150–60.
Bakkiyaraj D, Nandhini JR, Malathy B, Pandian SK. The anti-biofilm potential of pomegranate (Punica granatum L.) extract against human bacterial and fungal pathogens. Biofouling 2013;29(8):929–37.
Habibipour R, Moradi-Haghgou L, Farmany A. Green synthesis of AgNPs@ PPE and its Pseudomonas aeruginosa biofilm formation activity compared to pomegranate peel extract. Int J Nanomedicine 2019;14:6891–9.
Karami S, Roayaei M, Hamzavi H, Bahmani M, Hassanzad-Azar H, Leila M, et al. Isolation and identification of probiotic Lactobacillus from local dairy and evaluating their antagonistic effect on pathogens. Int J Pharm Investig 2017;7(3):137–41.
Mandal H, Jariwala R, Bagchi T. Isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics. Can J Microbiol 2016;62(4):349–59.
Hassanein KM, Agban MN, Nafie A, Abdelmoez NA. The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital. Egypt J Med Microbiol 2021;30(4):139–48.
Shokri D, Khorasgani MR, Mohkam M, Fatemi SM, Ghasemi Y, Taheri-Kafrani A. The Inhibition Effect of Lactobacilli Against Growth and Biofilm Formation of Pseudomonas aeruginosa. Probiotics Antimicrob Proteins 2018;10(1):34–42.
Asadzadegan R, Haratian N, Sadeghi M, Maroufizadeh S, Mobayen M, Sedigh Ebrahim Saraei H, et al. Antibiofilm and antimicrobial activity of Lactobacillus cell free supernatant against Pseudomonas aeruginosa isolated from burn wounds. Int Wound J 2023;20(10):4112–21.
Hoseinian M, Gholizadeh P, Ghotaslou A, Ghotaslou R. Lactobacillus spp. Inhibit the Biofilm Formation of Pseudomonas aeruginosa. Lett Appl NanoBioScience 2021;10(4):2843–9.
Lu L, Hu W, Tian Z, Yuan D, Yi G, Zhou Y, Cheng Q, Zhu J, Li M. Developing natural products as potential anti-biofilm agents. Chin Med 2019;14(1):1–7.
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