MICRO RNA 182-3-P, 519-D-5P, 378-3P AS NON-INVASIVE PREDICTORS OF PREECLAMPSIA

Authors

DOI:

https://doi.org/10.55519/JAMC-03-11783

Keywords:

Hypertension, microRNA, predictors, pregnancy, real time PCR

Abstract

Background: MicroRNAs (miRNAs) are an emerging field of interest in many diseases. Some of the miRNAs have been reported to be expressed differentially in diseased states of pregnancy. The current study was designed to measure and compare the levels of microRNA 182-3-p, 519-d-5p, and 378-3p and it was hypothesized that the microRNA 182-3-p, 519-d-5p, and 378-3p can be used as a non-invasive predictor of preeclampsia. Methods: Expression level of the miRNAs 182-3-p, 519-d-5p, and 378-3p was measured in the serum of preeclamptic and normal pregnancies by real-time PCR. Data was entered and analysed by Statistical Package for the Social Sciences 22 (SPSS). Results: Significantly high expression levels of MiRNA 182-3p, 519-d-5p and low levels of miR-378-3p were associated with preeclampsia (PE). Conclusion: The results revealed that miR-182-3p is a powerful predictor of PE with an Odds Ratio of 5.9 and can be used as a noninvasive, reliable predictor of PE to screen these patients at an early stage. Screening at early gestation with follow-up studies can emphasize the results

References

Friedman RC, Farh KK-H, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res 2009;19(1):92–105.

Ambros V. microRNAs: tiny regulators with great potential. Cell 2001;107(7):823–6.

Chen DB, Wang W. Human placental microRNAs and preeclampsia. Biol Reprod 2013;88(5):130,1–11.

Helige C, Ahammer H, Moser G, Hammer A, Dohr G, Huppertz B, et al. Distribution of decidual natural killer cells and macrophages in the neighbourhood of the trophoblast invasion front: a quantitative evaluation. Hum Reprod 2014;29(1):8–17.

Liu S, Diao L, Huang C, Li Y, Zeng Y, Kwak-Kim JY. The role of decidual immune cells on human pregnancy. J Reprod Immunol 2017;124:44–53.

Lash GE, Ernerudh J. Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage. J Reprod Immunol 2015;108:83–9.

Travaglino A, Raffone A, Saccone G, Migliorini S, Maruotti GM, Esposito G, et al. Placental morphology, apoptosis, angiogenesis and epithelial mechanisms in early-onset preeclampsia. Eur J Obstet Gynecol Reprod Biol 2019;234:200–6.

Yang S, Li H, Ge Q, Guo L, Chen F. Deregulated microRNA species in the plasma and placenta of patients with preeclampsia. Mol Med Rep 2015;12(1):527–34.

Munaut C, Tebache L, Blacher S, Noël A, Nisolle M, Chantraine F. Dysregulated circulating miRNAs in preeclampsia. Biomed Rep 2016;5(6):686–92.

Enquobahrie DA, Abetew DF, Sorensen TK, Willoughby D, Chidambaram K, Williams MA. Placental microRNA expression in pregnancies complicated by preeclampsia. Am J Obstet Gynecol 2011;204(2):178.e12–21.

Zhang Y, Diao Z, Su L, Sun H, Li R, Cui H, et al. MicroRNA-155 contributes to preeclampsia by down-regulating CYR61. Am J Obstet Gynecol 2010;202(5):466.e1–7.

Nadeem U, Ye G, Salem M, Peng C. MicroRNA-378a-5p targets cyclin G2 to inhibit fusion and differentiation in BeWo cells. Biol Reprod 2014;91(3):76,1–10.

Xie L, Mouillet JF, Chu T, Parks WT, Sadovsky E, Knöfler M, et al. C19MC microRNAs regulate the migration of human trophoblasts. Endocrinology 2014;155(12):4975–85.

Noack F, Ribbat-Idel J, Thorns C, Chiriac A, Axt-Fliedner R, Diedrich K, et al. miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia. J Perinat Med 2011;39(3):267–71.

Wu L, Zhou H, Lin H, Qi J, Zhu C, Gao Z, et al. Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies. Reproduction 2012;143(3):389–97.

Uzan J, Carbonnel M, Piconne O, Asmar R, Ayoubi JM. Pre-eclampsia: pathophysiology, diagnosis, and management. Vasc Health Risk Manag 2011;7:467–74.

Ali Z, Khaliq S, Zaki S, Ahmad HU, Lone KP. Differential expression of placental growth factor, transforming growth factor-β and soluble endoglin in peripheral mononuclear cells in preeclampsia. J Coll Physicians Surg Pak 2019;29(3):235–9.

Deslauriers L, McCarty LS, Miller K, Callaghan K, Kestin G. Measuring actual learning versus feeling of learning in response to being actively engaged in the classroom. Proc Natl Acad Sci 2019;116(39):19251–7.

Li Q, Long A, Jiang L, Cai L, Xie L, Gu JA, et al. Quantification of preeclampsia-related microRNAs in maternal serum. Biomed Rep 2015;3(6):792–6.

Xu H, Du Y, He J, Wang L, Sun G. MicroRNA-378 protects human umbilical vein endothelial cells from injuries by soluble CD226 through down-regulating the expression of soluble CD226 in natural killer cells. Biotechnol Biotechnol Equip 2019;33(1):1097–107.

Ali Z, Khaliq S. Hematological Markers: Emerging Diagnostic and Therapeutic Targets in Preeclampsia. Front Clin Drug Res-Hematol 2022;5:192.

Zhang S, Zhang Q, Shi G, Yin J. MiR-182-5p regulates BCL2L12 and BCL2 expression in acute myeloid leukemia as a potential therapeutic target. Biomed Pharmacother 2018;97:1189–94.

Fang Y, Huang Z, Li H, Tan W, Zhang Q, Wang L, et al. Highly expressed miR-182-5p can promote preeclampsia progression by degrading RND3 and inhibiting HTR-8/SVneo cell invasion. Eur Rev Med Pharmacol Sci 2018;22(20):6583–90.

Chaiwangyen W, Murrieta-Coxca JM, Favaro RR, Photini SM, Gutiérrez-Samudio RN, Schleussner E, et al. miR-519d-3p in trophoblastic cells: effects, targets and transfer to allogeneic immune cells via extracellular vesicles. Int J Mol Sci 2020;21(10):3458.

Su S, Yang F, Zhong L, Pang L. Circulating noncoding RNAs as early predictive biomarkers in preeclampsia: a diagnostic meta-analysis. Reprod Biol Endocrinol 2021;19(1):177.

Additional Files

Published

2023-08-10